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SUMMARY:Exploring and Exploiting the Diversity of Secretory Routes in Mamm
 alian Cells - Franck Perez
DTSTART:20171128T140000Z
DTEND:20171128T150000Z
UID:TALK86081@talks.cam.ac.uk
CONTACT:Scientific Meetings Co-ordinator
DESCRIPTION:It is now clear that multiple routes exist in cells to transpo
 rt proteins and lipids toward various compartments. To analyse these route
 s\, there is a strong a need for a versatile\, highly synchronized assay\,
  usable at physiological temperature in living cells. We developed the RUS
 H system (Retention Using Selective Hooks)\, a two-state assay based on th
 e reversible interaction of a Reporter protein with a Hook protein stably 
 localized in a donor compartment. This system can be used to study and qua
 ntify the trafficking of very diverse proteins of different size\, shape a
 nd destined to various compartments. The RUSH system can be used for real 
 time monitoring of transport in living cells as well as for for High Throu
 ghput screening using automated imaging.\n\nWe are using the RUSH assay at
  multiple scales to monitor the transport of proteins in cells and analyse
  their intracellular signatures\, to map the diversity of trafficking rout
 es in terms of biochemical composition\, to integrate these routes with ot
 her cellular machineries\, like cellular adhesion and polarization\, and t
 o screen diverse chemical libraries and identify potential therapeutic mol
 ecules. 
LOCATION:Max Perutz Lecture Theatre\, Medical Research Council (MRC) (MRC 
 Laboratory of Molecular Biol
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