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SUMMARY:Deciphering the origins of gene expression bursts at dawn\, using 
 computer-aided experimental design - Dr Daphne Ezer\, Sainsbury Laboratory
 \, University of Cambridge
DTSTART:20171129T140000Z
DTEND:20171129T150000Z
UID:TALK93412@talks.cam.ac.uk
CONTACT:27743
DESCRIPTION:Abstract: Eukaryotes are able to change gene expression within
  minutes\, but it is difficult to identify regulatory targets for genes th
 at are only expressed for a very short length of time.   To help resolve t
 hese regulatory relationships\, we sought to create a high-resolution time
  course to capture these transient expression changes\, but also obtain a 
 large number of genetic and environmental perturbation experiments to disc
 riminate between causative and correlative relationships between genes.  B
 ecause the large number of samples required for a high resolution time cou
 rse under many conditions rapidly becomes unpractical\, we have created a 
 general-purpose tool\, New Investigation Time Point Picker (NITPicker)\, t
 o aide in the design of experiments that require both fine-scale temporal 
 resolution and many perturbation experiments.  NITPicker identifies subset
 s of time points within a high resolution time course which would be most 
 informative to sample in perturbation experiments\, taking into account la
 b budget and experimental requirements.  In particular\, we apply this too
 l to help identify the how plants rapidly adjust their transcriptomes at t
 he onset of dawn\, when the circadian clock is entrained by light and when
  plants are most resilient to many environmental stressors.   By using a h
 igh-resolution RNA-seq time course (sampled every 2 minutes)\, as well as 
 RNA-seq time course experiments in which the circadian clock\, light perce
 ption\, and temperature response are perturbed\, we determined that there 
 are numerous waves of gene expression after dawn\, and that plants employ 
 a 'multi-threading' approach to coordinate environmental and circadian sig
 nals. 
LOCATION:MR4\, Centre for Mathematical Sciences\, Wilberforce Road\, Cambr
 idge
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