BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:Ribosome profiling and virus infection - Dr Andrew Firth\, Departm
 ent of Pathology
DTSTART:20180221T140000Z
DTEND:20180221T150000Z
UID:TALK93427@talks.cam.ac.uk
CONTACT:27743
DESCRIPTION:Abstract: While standard RNA-Seq experiments can be used to mo
 nitor the abundance of every messenger RNA (mRNA) species in a cell\, this
  is only an inaccurate proxy for actual levels of protein synthesis\, sinc
 e different mRNAs can have strikingly different translation efficiencies. 
 Further\, mRNA translation efficiency can vary in response to environmenta
 l and developmental stimuli. In contrast\, Ribosome Profiling (Ribo-Seq) p
 rovides a direct measurement of protein synthesis. Ribosome profiling reli
 es on the fact that a translating ribosome protects ~30 nucleotides of mRN
 A. An RNA nuclease is used to digest unprotected RNA\, the 30-nt ribosome-
 protected fragments (RPFs) are purified for deep sequencing\, and the sequ
 enced fragments are mapped back to the transcriptome\, to give a global sn
 apshot of protein synthesis in the cell. Ribosome profiling has proven to 
 be increasingly valuable in studies of the translation process\, for examp
 le\, in the discovery of novel translated open reading frames (ORFs)\, det
 ermination of elongation rates\, and identification of sites of ribosome p
 ausing. It also has broad application in the analysis of global protein sy
 nthesis and has been exploited in studies of infectious diseases\, cell gr
 owth\, differentiation and development\, mitochondrial gene expression\, a
 nd cell stress. In collaboration with the Brierley lab (http://www.path.ca
 m.ac.uk/directory/ian-brierley) we have been applying Ribo-Seq and paralle
 l RNA-seq to a variety of virus species to study the kinetics of virus gen
 e expression\, unusual transcriptional and translational mechanisms employ
 ed by viruses\, and host responses to virus infection at both the transcri
 ptional and translational levels. 
LOCATION:MR4\, Centre for Mathematical Sciences\, Wilberforce Road\, Cambr
 idge
END:VEVENT
END:VCALENDAR