BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Talks.cam//talks.cam.ac.uk//
X-WR-CALNAME:Talks.cam
BEGIN:VEVENT
SUMMARY:RNA Genomics in Health and Disease - Robert B Darnell\, MD PhD\, T
 he Rockefeller University and Howard Hughes Medical Institute
DTSTART:20180412T120000Z
DTEND:20180412T130000Z
UID:TALK95113@talks.cam.ac.uk
CONTACT:Kate Davenport
DESCRIPTION:Cell-specific RNA CLIP\n\nOur laboratory has discovered that n
 eurons have unique systems for regulating RNA metabolism that are used by 
 neurons.  These neuron-specific RNA binding proteins are encoded by multig
 ene families that encode such regulatory factors as Nova\, nElavl\, Mbnl a
 nd RbFox (NeuN).  We developed the CLIP method to precisely map RNA-protei
 n interaction sites in living brain or other tissues with single nucleotid
 e resolution.  This has led to new understanding of the function of these 
 proteins in vivo.  CLIP is a general method\, and we have expanded it to t
 he study of the Fragile-X protein FMRP and Argonaute-microRNA-mRNA ternary
  interactions\, allowing identification of translational control and micro
 RNA binding sites on a transcriptome-wide scale.  We will discuss more rec
 ent explorations of CLIP in functional annotation of the transcriptome\, a
 nd a new platform method to undertake CLIP studies in single cell types wi
 thin the brain.  The increasing resolution of CLIP allows new insights int
 o cell-specific roles of RNA regulation in specific paradigms of normal an
 d abnormal cellular physiology.\n\nSelect References (past decade)\nLicata
 losi et al. Nature 456:464-9\, 2008\; Chi et al.\, Nature 460:479-86\, 200
 9\; Zhang et al.\, Science 329:439-443\, 2010\, Darnell et al.\, Cell 146:
 247-61\, 2011\; Ince-Dunn et al.\, Neuron 75:437-450\, 2012\; Darnell\, An
 n Rev Neurosci 36:243-70\, 2013\; Vanharanta et al.\, eLife 4:e02734\, 201
 4\; Moore et al.\, Nature Comm 6:8864-81\, 2015\; Luna et al.\, Cell 160:1
 099-1110\, 2015\; Mol Cell 67:400-410\, 2017\; Hwang et al.\, Cell Reports
  15:423-435\, 2016\; Neuron 95:1334-1349\, 2017\; Jereb et al.\, eLife\, 2
 018 Mar 26\;7. pii: e34042\; Yuan Y et al.\, Darnell RB. BioRxiv 237347\; 
 doi: https://doi.org/10.1101/237347\, 2018.\n\n
LOCATION:CRUK CI Lecture Theatre
END:VEVENT
END:VCALENDAR