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SUMMARY:Using NanoBiT® Technology to Study Protein-Protein Interactions i
 n Living Cells - Mr. Andrei Smid (Dept of Medicine) &amp\;  Dr. Jacek Mokr
 osinski (IMS)
DTSTART:20180124T183000Z
DTEND:20180124T200000Z
UID:TALK96553@talks.cam.ac.uk
CONTACT:Postdoc Masterclass
DESCRIPTION:G-protein coupled receptors (GPCRs) respond to various extrace
 llular stimuli\, activating numerous intracellular signaling pathways. The
 y are the largest protein family\, and 100+ are drug targets for 30% of av
 ailable drugs. A better understanding of signal transduction mechanisms an
 d the physiological role of GPCRs can aid further drug discovery. While st
 udying signal transduction dynamics remains a challenge\, the speakers wil
 l explain how NanoBiT® technology allows the development of new assays to
  study molecular mechanisms of cell signaling. \nThe first speaker will di
 scuss the NanoBiT® protein-protein interaction assay for monitoring pre-T
 -cell receptor clustering and how fluorophore tagging of luciferase compon
 ents enables easy normalisation of luminous signals\, with the application
  of the assay in microscopy. The second speaker will evaluate these method
 s in the Genetics of Obesity Study\, exploring how genetic variants identi
 fied in patients with severe early onset obesity affect signaling pathways
  that regulate appetite and energy expenditure.\n
LOCATION: Postdoc Centre\, Biomedical Campus\, Bay 13\, Hills Road\, Cambr
 idge CB2 0SP
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