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SUMMARY:Exploring the mechanisms of  haematopoietic lineage progression at
  the single-cell level - Dr Ana Cvejic - The Sanger Centre &amp\; Dept of 
 Haematology\, Cambridge.
DTSTART:20180221T161500Z
DTEND:20180221T171500Z
UID:TALK98443@talks.cam.ac.uk
CONTACT:David Greaves
DESCRIPTION:The success of marker-based approaches for dissecting haematop
 oiesis in mouse and human is reliant on the presence of well-defined cell-
 surface markers specific for diverse progenitor populations. An inherent p
 roblem with this approach is that the presence of specific cell surface ma
 rkers does not directly reflect the transcriptional state of a cell. Here 
 we used a marker-free approach to computationally reconstruct the blood li
 neage tree in zebrafish and order cells along their differentiation trajec
 tory\, based on their global transcriptional differences. Within the popul
 ation of transcriptionally similar stem and progenitor cells our analysis 
 revealed considerable cell-to-cell differences in their probability to tra
 nsition to another\, committed state. Once fate decision was executed\, th
 e suppression of transcription of ribosomal genes and up-regulation of lin
 eage specific factors coordinately controlled lineage differentiation. Evo
 lutionary analysis further demonstrated that this haematopoietic program w
 as highly conserved between zebrafish and higher vertebrates.\n
LOCATION:Lecture Theatre 1\, Computer Laboratory
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