Abstract

Spatially mapped biomarker information at the single-cell level is crucial to understanding cellular organization and cell-to-cell interactions in complex tissue environment, and understanding cellular interactions within gene and cell therapy models. RNAscope® and BaseScope™ assays, the two highly specific and sensitive ISH methods allow visualizing gene expression and genetic variations in tissue environments. This seminar will highlight: • Detection of mRNAs, viral RNAs, and lncRNAs at single molecule sensitivity by RNAscope® assay • Use as cell markers for cell therapy T-cell and viral vector localization as a sensitive safety assessment tool • Validation of target gene expression after high-throughput transcriptome analysis • Target specific splice junctions by BaseScope™ assay • Distinguish specific homologues, paralogues, and orthologues by RNAscope® or BaseScope™ assays • Perform single cell analysis within tissue architecture in FFPE or frozen tissues