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Phenotyping iPS-CM's with light

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We lack the ability to study cardiomyocyte form (sarcomere spacing, orientation, density) and function (calcium, voltage, force) in living cells. This is especially true of the amorphous IPS derivative at single cell scale. I will show how, and explain why existing dye based approaches cannot be considered suitable partners in studies of contractility. Instead, we make proteins to reveal IPS -CM form and function in real-time, in the absence of contractile impairment, and in combination with quantitative force measurement, even when buried in the myofilament itself.

This talk is part of the Cambridge Cardiovascular Seminar Series series.

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