Abstract

The Ly group focuses on two main areas: 1) the technological development of mass spectrometry-based proteomics to comprehensively measure protein parameters in rare, transient cell states and 2) the control of cell state transitions, including the quiescence to proliferation switch and mitotic entry. Previously, we developed a method to perform proteomics of intracellular immunostained subsets, which we call PRIMMUS (Ly et al., eLife 2017). The PRIMMUS approach avoids potential artefacts associated with inhibitor-based synchronisation and allows for high resolution purification and analysis of specific, transiently populated cell cycle states. In my talk, I will present recent, unpublished advances that enable proteome analysis of 2,000 human lymphoblastoid cells to a depth of ~5,000 proteins. Additionally, we have obtained promising preliminary results applying PRIMMUS to measure proteins in single cells. Our aim is to apply this workflow to explore cell-type specificity in proliferation regulation by comparing the cell cycle regulated network between human cell types.