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Watching Individual Proteins Working on Single Molecules of DNA: from Biophysics to Cancer

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We can now watch individual proteins acting on single molecules of DNA . Visualization is achieved by capturing a single DNA molecule with optical traps or by tethering to a glass surface. Proteins are visualized via fluorescent reporters, and molecules are manipulated using microfluidic flowcells. Using these approaches, we have imaged proteins functioning in the homology-directed repair of DNA breaks. We can image the search for DNA homology conducted by the RecA, the self-assembly of RecA and RAD51 on DNA , and the function of mediators such as RecFOR and BRCA2 .

This talk is part of the MRC LMB Seminar Series series.

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Seminar time is 1pm BST in Room FW 11, Willam Gates Hall. Zoom link: https://cl-cam-ac-uk.zoom.us/j/4361570789?pwd=Nkl2T3ZLaTZwRm05bzRTOUUxY3Q4QT09&from=addon https://zoom.us/j/94218164135 cripsr

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