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Study and characterization of MET1 homologues in Arabidopsis thaliana

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1st year graduate talk

Epigenetic marks are of high importance for gene regulation. The most studied epigenetic mark is DNA methylation that is mainly associated with gene silencing in plant and mammals. Arabidopsis thaliana mutants in the gene encoding the main methyltransferase (MET1) show global hypomethylation and ectopic expression of genes and transposable elements, leading to serious phenotypic consequences. DNA of met1 mutants loses almost all of their CG methylation and this effect cannot be reverted by reintroducing wild-type version of MET1 gene. The functions of MET1 homologues MET 2 , MET3 and MET4 are still unknown to this date but have been hypothesized to influence endosperm imprinting. In my research, I propose to characterise phenotypes and DNA methylation levels of the combinations of mutants of MET1 homologues in Arabidopsis thaliana to unveil their functions.

This talk is part of the Plant Sciences Departmental Seminars series.

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