Abstract

The CRISPR /Cas9 genome editing technology is a very powerful tool that is being used extensively in labs around the world. This adapted immunity system from bacteria works in a simple way to cleave DNA . In its original, wild-type format, CRISPR /Cas9 can create double stranded breaks in DNA but the nuclease enzyme can also selectively cleave a single DNA strand or bind to a specific region of interest. The most common use of this technology is to reduce the level of a target protein by introducing a stop codon in the mRNA. However, further uses in modifying epigenetic features of DNA and introducing or deleting DNA sequences, have also been explored. Controversially, CRISPR /Cas9 has been used to modify the genome of human tripronuclear zygotes and debates regarding the ethics of this are ongoing. In this masterclass, the speakers will give an overview of the CRISPR /Cas9 technology, including its many uses and their experiences of this system.