Abstract

Recent discoveries have enabled transmission into the gas phase of membrane complexes from detergent micelles in solution. By maintaining interactions between membrane and cytoplasmic subunits in the mass spectrometer, it is now possible to investigate the effects of lipids, nucleotides and drugs on intact membrane assemblies. These investigations reveal allosteric and synergistic effects of small molecule binding and expose the consequences of post-translational modifications. Whilst very insightful, the choice of detergent can sometimes perturb interactions within the membrane leading to changes in the lipid environment, loss of small molecules and disruption of protein interactions. In my lecture, I will present recent progress in our quest to eject complexes directly from membrane mimetics or native membranes and to fragment the small molecules harboured within them.

Join the webinar live using this link – https://zoom.us/j/91399302621